Immune expression profile identification in a group of proliferative verrucous leukoplakia patients: a pre-cancer niche for oral squamous cell carcinoma development
Por:
Llorens C, Soriano B, Trilla-Fuertes L, Bagan L, Ramos-Ruiz R, Gamez-Pozo A, Pena C, Bagan J
Publicada:
1 sep 2020
Ahead of Print:
1 sep 2020
Resumen:
Objectives To explore the pathophysiology of proliferative verrucous leukoplakia, a rare oral disorder that exhibits high rates of recurrence and malignant transformation, through a RNAseq case-control study. Material and methods We obtained oral biopsies from 10 patients with verrucous leukoplakia lesions and from the mucosa of 5 healthy individuals for sequencing using RNAseq technology. Using bioinformatic methods, we investigated gene expression and enrichment differences between patients both with and without the disorder. We applied network biology methods to investigate functional relations among those genes that were differentially deregulated. Results We detected 140 differentially expressed genes with distinct roles in immune surveillance, tissue and organ morphogenesis, development, and organization. Of these 140 genes, 111 have been previously described as cancer expression biomarkers, being oral squamous cell carcinoma the most represented type of cancer among them. Of these 140 genes, 26 were prioritized for further investigation as biomarkers using larger sample sizes. Conclusions The gene expression patterns of healthy and unhealthy patients differed in 140 genes whose deregulation has a functional impact on normal functioning of the immune system. This immune expression profile provides a plausible hypothesis to explain the transformation to oral squamous cell carcinoma observed in 6 of the 10 assayed cases.
Filiaciones:
Llorens C:
Univ Valencia, Biotechvana, Parc Cient, Valencia, Spain
Soriano B:
Univ Valencia, Biotechvana, Parc Cient, Valencia, Spain
Trilla-Fuertes L:
Biomed Mol Med SL, Madrid, Spain
Bagan L:
Univ Valencia, Univ Gen Hosp, Med Oral Unit, Valencia, Spain
Ramos-Ruiz R:
Parque Cient Madrid, Genom Unit Cantoblanco, Madrid, Spain
Gamez-Pozo A:
Hosp Univ La Paz IdiPAZ, Mol Oncol & Pathol Lab, Madrid, Spain
Pena C:
CIBERONC, Inst Ramon y Cajal Invest Sanitaria IRYCIS, Med Oncol Dept, Madrid, Spain
Inst Ramon y Cajal Invest Sanitaria IRYCIS, Lab Invest Oncol Med, Planta 2 Dcha,Carretera Colmenar,Km 9,100, Madrid 28034, Spain
Bagan J:
Univ Valencia, Univ Gen Hosp, Med Oral Unit, Valencia, Spain
Med Bucal Univ Valencia, Hosp Gen Univ Valencia, Serv Estomatol CMF, Fdn Invest, Valencia, Spain
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