The surface charge of electroactive materials governs cell behaviour through its effect on protein deposition
Por:
Rodriguez-Lejarraga P, Martin-Iglesias S, Moneo-Corcuera A, Colom A, Redondo-Morata L, Giannotti MI, Petrenko V, Monleón-Guinot I, Mata M, Silvan U, Lanceros-Mendez S
Publicada:
1 ago 2024
Ahead of Print:
1 jul 2024
Resumen:
The precise mechanisms underlying the cellular response to static electric cues remain unclear, limiting the design and development of biomaterials that utilize this parameter to enhance specific biological behaviours. To gather information on this matter we have explored the interaction of collagen type-I, the most abundant mammalian extracellular protein, with poly(vinylidene fluoride) (PVDF), an electroactive polymer with great potential for tissue engineering applications. Our results reveal significant differences in collagen affinity, conformation, and interaction strength depending on the electric charge of the PVDF surface, which subsequently affects the behaviour of mesenchymal stem cells seeded on them. These findings highlight the importance of surface charge in the establishment of the material-protein interface and ultimately in the biological response to the material. The development of new tissue engineering strategies relies heavily on the understanding of how biomaterials interact with biological tissues. Although several factors drive this process and their driving principles have been identified, the relevance and mechanism by which the surface potential influences cell behaviour is still unknown. In our study, we investigate the interaction between collagen, the most abundant component of the extracellular matrix, and poly(vinylidene fluoride) with varying surface charges. Our findings reveal substantial variations in the binding forces, structure and adhesion of collagen on the different surfaces, which collectively explain the differential cellular responses. By exposing these differences, our research fills a critical knowledge gap and paves the way for innovations in material design for advanced tissue regeneration strategies. (c) 2024 Acta Materialia Inc. Published by Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
Filiaciones:
Rodriguez-Lejarraga P:
BCMaterials, Basque Center for Materials, Applications and Nanostructures, 48940 Leioa, Spain
Martin-Iglesias S:
BCMaterials, Basque Center for Materials, Applications and Nanostructures, 48940 Leioa, Spain
Moneo-Corcuera A:
BCMaterials, Basque Center for Materials, Applications and Nanostructures, 48940 Leioa, Spain
Colom A:
Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain
Biofisika Institute (CSIC, UPV/EHU), 48940 Leioa, Spain
Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, Campus Universitario, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain
Redondo-Morata L:
Université de Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017, CIIL-Center for Infection and Immunity of Lille, F-59000 Lille, France
Giannotti MI:
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute for Science and Technology, 08028 Barcelona, Spain
CIBER-BBN, ISCIII, 08028 Barcelona, Spain
Department of Materials Science and Physical Chemistry, University of Barcelona, Martí i Franquès 10, 08028 Barcelona, Spain
Petrenko V:
BCMaterials, Basque Center for Materials, Applications and Nanostructures, 48940 Leioa, Spain
Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain
Monleón-Guinot I:
Department of Pathology, Faculty of Medicine and Dentistry, Universitat de València, 46010 Valencia, Spain
INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Mata M:
Department of Pathology, Faculty of Medicine and Dentistry, Universitat de València, 46010 Valencia, Spain
INCLIVA Biomedical Research Institute, 46010 Valencia, Spain
Silvan U:
BCMaterials, Basque Center for Materials, Applications and Nanostructures, 48940 Leioa, Spain
Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain
Lanceros-Mendez S:
BCMaterials, Basque Center for Materials, Applications and Nanostructures, 48940 Leioa, Spain
Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain
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