Complex effects of Mg-biomaterials on the osteoblast cell machinery: A proteomic study
Por:
Cerqueira A, García-Arnáez I, Romero-Gavilán F, Azkargorta M, Elortza F, de Llanos J, Carda C, Gurruchaga M, Goñi I, Suay J
Publicada:
1 jun 2022
Ahead of Print:
1 may 2022
Resumen:
The cell-biomaterial interface is highly complex; thousands of molecules and many processes participate in its formation. Growing demand for improved biomaterials has highlighted the need to understand the structure and functions of this interface. Proteomic methods offer a viable alternative to the traditional in vitro techniques for analyzing such systems. Magnesium is a promoter of cell adhesion and osteogenesis. Here, we used the LC-MS/MS to compare the protein expression profiles of human osteoblasts (HOb) exposed to sol-gel coatings without (MT) and with Mg (MT1.5Mg) for 1, 3, and 7 days. PANTHER, DAVID, and IPA databases were employed for protein identification and data analysis. Confocal microscopy and gene expression analysis were used for further characterization. Exposure to MT1.5Mg increased the HOb cell area and the expression of SP7, RUNX2, IBP3, COL3A1, MXRA8, and FBNI genes. Proteomic analysis showed that MT1.5Mg affected the early osteoblast maturation (PI3/AKT, mTOR, ERK/MAPK), insulin metabolism, cell adhesion (integrin, FAK, actin cytoskeleton regulation) and oxidative stress pathways. Thus, the effects of Mg on cell adhesion and osteogenesis are rather complex, affecting several pathways rather than single processes. Our analysis also confirms the potential of proteomics in biomaterial characterization, showing a good correlation with in vitro results.
Filiaciones:
Cerqueira A:
Univ Jaume 1, Dept Ind Syst Engn & Design, Av Vicent Sos Baynat S-N, Castellon De La Plana 12071, Spain
García-Arnáez I:
Univ Basque Country, Dept Sci & Technol Polymers, PM de Lardizabal 3, San Sebastian 20018, Spain
Romero-Gavilán F:
Univ Jaume 1, Dept Ind Syst Engn & Design, Av Vicent Sos Baynat S-N, Castellon De La Plana 12071, Spain
Azkargorta M:
ProteoRed ISCIII, Basque Res & Technol Alliance BRTA, CIC BioGUNE, Prote Platform, Bizkaia Sci & Technol Pk, Derio 48160, Spain
Elortza F:
ProteoRed ISCIII, Basque Res & Technol Alliance BRTA, CIC BioGUNE, Prote Platform, Bizkaia Sci & Technol Pk, Derio 48160, Spain
de Llanos J:
Univ Valencia, Med Fac, Dept Pathol Med & Odontol, Av Blasco Ibanez 13, Valencia 46010, Spain
Univ Clin Hosp Valencia INCLIVA, Res Inst, C de Menendez y Pelayo 4, Valencia 46010, Spain
Carda C:
Univ Valencia, Med Fac, Dept Pathol Med & Odontol, Av Blasco Ibanez 13, Valencia 46010, Spain
Univ Clin Hosp Valencia INCLIVA, Res Inst, C de Menendez y Pelayo 4, Valencia 46010, Spain
Gurruchaga M:
Univ Basque Country, Dept Sci & Technol Polymers, PM de Lardizabal 3, San Sebastian 20018, Spain
Goñi I:
Univ Basque Country, Dept Sci & Technol Polymers, PM de Lardizabal 3, San Sebastian 20018, Spain
Suay J:
Univ Jaume 1, Dept Ind Syst Engn & Design, Av Vicent Sos Baynat S-N, Castellon De La Plana 12071, Spain
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