Different Mutational Profiles of Subcutaneous Panniculitis-like T-cell Lymphoma and Lupus Panniculitis: An Additional Case Series.


Por: Machan S, Rodríguez M, Manso R, Borregón J, Chamizo C, Alonso-Alonso R, Rodríguez-Peralto JL, Torres Nieto MÁ, Monteagudo C, García Toro E, Cerroni L, García C, Estrach T, García Herrera A, Ferrer B, García-Patos V, Segues N, Díaz de la Pinta FJ, Afonso-Martin JL, Peñate Y, Limeres-Gonzalez MÁ, González-Núñez MÁ, González-Cruz C, García Fernández E, Cereceda L, Minguez P, de la Fuente L, Requena L, Rodríguez-Pinilla SM

Ahead of Print: 18 jul 2024
Resumen:
BACKGROUND: Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) is a rare cytotoxic T-cell lymphoma with indolent behavior, mostly present in women and associated with immunological diseases whose pathogenic background is still poorly understood. SPTCL is associated with lupus erythematosus panniculitis (LEP) and histologically misdiagnosed. OBJECTIVES: The aim of our study was to identify mutations affecting the pathogenesis of both SPTCL and LEP. MATERIALS AND METHODS: We studied a total of 10 SPTCL and 10 LEP patients using targeted next-generation sequencing and pyrosequencing. Differences in gene expression between molecular subgroups were investigated using NanoString technology. Clinical data were collected, and correlations sought with the molecular data obtained. RESULTS: The mutational profile of SPTCL and LEP is different. We identified fewer pathogenic mutations than previously reported in SPTCL, noting a single HAVCR2-mutated SPTCL case. Interestingly, 40% of our SPTCL cases showed the pathogenic TP53 (p.Pro72Arg) (P72R) variant. Although cases showing HAVCR2 mutations or the TP53 (P72R) variant had more severe symptomatic disease, none developed hemophagocytic syndrome (HPS). Furthermore, TP53 (P72R)-positive cases were characterized by a lower metabolic signaling pathway and higher levels of CD28 expression and Treg signaling genes. In addition, 30% of our cases featured the same mutation (T735C) of the epigenetic modificatory gene DNMT3A. None of the LEP cases showed mutations in any of the studied genes. CONCLUSIONS: The mutational landscape of SPTCL is broader than previously anticipated. We describe, for the first time, the involvement of the TP53 (P72R) pathogenic variant in this subgroup of tumors, consider the possible role of different genetic backgrounds in the development of SPTCL, and conclude that LEP does not follow the same pathogenic pathway as SPTCL.

Filiaciones:
Machan S:
 Department of Dermatology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

Rodríguez M:
 Department of Pathology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

 CIBERONC (Centro de Investigación Biomédica en Red de Cáncer), Spain

Manso R:
 Department of Pathology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

 CIBERONC (Centro de Investigación Biomédica en Red de Cáncer), Spain

Borregón J:
 Department of Pathology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

 CIBERONC (Centro de Investigación Biomédica en Red de Cáncer), Spain

Chamizo C:
 Department of Pathology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

 CIBERONC (Centro de Investigación Biomédica en Red de Cáncer), Spain

Alonso-Alonso R:
 Department of Pathology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

 CIBERONC (Centro de Investigación Biomédica en Red de Cáncer), Spain

Rodríguez-Peralto JL:
 Hospital Universitario 12 de Octubre, Madrid, Spain

Torres Nieto MÁ:
 Hospital Universitario Río Hortega, Valladolid, Spain

Monteagudo C:
 Hospital Clinico Universitario de Valencia, Universidad de Valencia, Valencia, Spain

García Toro E:
 Hospital Universitario de Burgos, Burgos, Spain

Cerroni L:
 Dermatopathology Research Unit, Department of Dermatology, Medical University of Graz, Graz, Austria

García C:
 Hospital Universitario de Canarias, Tenerife, Spain

Estrach T:
 Hospital Clínic de Barcelona, Barcelona, Spain

García Herrera A:
 Hospital Clínic de Barcelona, Barcelona, Spain

Ferrer B:
 Hospital Vall d'Hebron, Barcelona, Spain

García-Patos V:
 Hospital Vall d'Hebron, Barcelona, Spain

Segues N:
 Hospital Universitario Donostia, San Sebastián, Spain

Díaz de la Pinta FJ:
 Department of Pathology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

 CIBERONC (Centro de Investigación Biomédica en Red de Cáncer), Spain

Afonso-Martin JL:
 Complejo Hospitalario Universitario Insular Materno-Infantil, Las Palmas de Gran Canaria, Spain

Peñate Y:
 Complejo Hospitalario Universitario Insular Materno-Infantil, Las Palmas de Gran Canaria, Spain

Limeres-Gonzalez MÁ:
 Hospital Universitario de Gran Canaria Doctor Negrín, Las Palmas de Gran Canaria, Spain

González-Núñez MÁ:
 Hospital Ciudad de Coria y Hospital San Pedro de Alcántara, Cáceres, Spain

González-Cruz C:
 Hospital Vall d'Hebron, Barcelona, Spain

García Fernández E:
 Department of Hematology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

Cereceda L:
 Department of Pathology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

 CIBERONC (Centro de Investigación Biomédica en Red de Cáncer), Spain

Minguez P:
 Department of Genetics, Instituto de Investigación Sanitaria - Fundación Jiménez Díaz University Hospital, Universidad Autónoma de Madrid (IIS-FJD, UAM), Madrid, Spain

 Bioinformatics Unit, Instituto de Investigación Sanitaria - Fundación Jiménez Díaz University Hospital, Universidad Autónoma de Madrid (IIS-FJD, UAM), Madrid, Spain

 Center for Biomedical Network Research on Rare Diseases (CIBERER), ISCIII, Madrid, Spain

de la Fuente L:
 Department of Genetics, Instituto de Investigación Sanitaria - Fundación Jiménez Díaz University Hospital, Universidad Autónoma de Madrid (IIS-FJD, UAM), Madrid, Spain

 Bioinformatics Unit, Instituto de Investigación Sanitaria - Fundación Jiménez Díaz University Hospital, Universidad Autónoma de Madrid (IIS-FJD, UAM), Madrid, Spain

Requena L:
 Department of Dermatology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

Rodríguez-Pinilla SM:
 Department of Pathology, Fundación Jiménez Díaz-IIS, Universidad Autónoma de Madrid, Madrid, Spain

 CIBERONC (Centro de Investigación Biomédica en Red de Cáncer), Spain
ISSN: 00017310





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ELSEVIER ESPANA S I, INFANTA MERCEDES 90, MADRID, 28020, SPAIN, España
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WOS Id: 001439025200001
ID de PubMed: 39032781

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