Concordance in the estimation of tumor percentage in non-small cell lung cancer using digital pathology
Por:
Carretero-Barrio I, Pijuan L, Illarramendi A, Curto D, López-Ríos F, Estébanez-Gallo Á, Castellvi J, Granados-Aparici S, Compañ-Quilis D, Noguera R, Esteban-Rodríguez I, Sánchez-Güerri I, Ramos-Guerra AD, Ortuño JE, Garrido P, Ledesma-Carbayo MJ, Benito A, Palacios J
Publicada:
15 oct 2024
Ahead of Print:
15 oct 2024
Resumen:
The incorporation of digital pathology in clinical practice will require the training of pathologists in digital skills. Our study aimed to assess the reliability among pathologists in determining tumor percentage in whole slide images (WSI) of non-small cell lung cancer (NSCLC) using digital image analysis, and study how the results correlate with the molecular findings. Pathologists from nine centers were trained to quantify epithelial tumor cells, tumor-associated stromal cells, and non-neoplastic cells from NSCLC WSI using QuPath. Then, we conducted two consecutive ring trials. In the first trial, analyzing four WSI, reliability between pathologists in the assessment of tumor cell percentage was poor (intraclass correlation coefficient (ICC) 0.09). After performing the first ring trial pathologists received feedback. The second trial, comprising 10 WSI with paired next-generation sequencing results, also showed poor reliability (ICC 0.24). Cases near the recommended 20% visual threshold for molecular techniques exhibited higher values with digital analysis. In the second ring trial reliability slightly improved and human errors were reduced from 5.6% to 1.25%. Most discrepancies arose from subjective tasks, such as the annotation process, suggesting potential improvement with future artificial intelligence solutions.
Filiaciones:
Carretero-Barrio I:
Department of Pathology, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034, Madrid, Spain
Faculty of Medicine, Universidad de Alcalá, 28801, Alcalá de Henares, Spain
CIBERONC, 28029, Madrid, Spain
Pijuan L:
Department of Pathology, Hospital Universitari Bellvitge, L'Hospitalet de Llobregat, 08097, Barcelona, Spain
Illarramendi A:
Department of Pathology, Hospital Universitario 12 de Octubre, 28041, Madrid, Spain
Curto D:
Department of Pathology, Hospital Universitario 12 de Octubre, 28041, Madrid, Spain
López-Ríos F:
CIBERONC, 28029, Madrid, Spain
Department of Pathology, Hospital Universitario 12 de Octubre, 28041, Madrid, Spain
Estébanez-Gallo Á:
Department of Pathology, Hospital Universitario Marqués de Valdecilla, 39011, Santander, Spain
Castellvi J:
CIBERONC, 28029, Madrid, Spain
Department of Pathology, Hospital Universitario Vall D'Hebron, 08035, Barcelona, Spain
Granados-Aparici S:
CIBERONC, 28029, Madrid, Spain
Department of Pathology, Medical School, University of Valencia-INCLIVA, 46010, Valencia, Spain
Compañ-Quilis D:
Department of Pathology, Hospital Clínico Universitario de Valencia, 46010, Valencia, Spain
Noguera R:
CIBERONC, 28029, Madrid, Spain
Department of Pathology, Medical School, University of Valencia-INCLIVA, 46010, Valencia, Spain
Esteban-Rodríguez I:
Department of Pathology, Hospital Universitario La Paz, 28046, Madrid, Spain
Sánchez-Güerri I:
Department of Pathology, Hospital del Mar, 08003, Barcelona, Spain
Ramos-Guerra AD:
CIBER-BBN, ISCIII, 28029, Madrid, Spain
Biomedical Image Technologies, ETSI Telecomunicación, Universidad Politécnica de Madrid, 28040, Madrid, Spain
Ortuño JE:
CIBER-BBN, ISCIII, 28029, Madrid, Spain
Biomedical Image Technologies, ETSI Telecomunicación, Universidad Politécnica de Madrid, 28040, Madrid, Spain
Garrido P:
Faculty of Medicine, Universidad de Alcalá, 28801, Alcalá de Henares, Spain
CIBERONC, 28029, Madrid, Spain
Medical Oncology Department, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034, Madrid, Spain
Ledesma-Carbayo MJ:
CIBER-BBN, ISCIII, 28029, Madrid, Spain
Biomedical Image Technologies, ETSI Telecomunicación, Universidad Politécnica de Madrid, 28040, Madrid, Spain
Benito A:
Department of Pathology, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034, Madrid, Spain
Faculty of Medicine, Universidad de Alcalá, 28801, Alcalá de Henares, Spain
Palacios J:
Department of Pathology, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034, Madrid, Spain
Faculty of Medicine, Universidad de Alcalá, 28801, Alcalá de Henares, Spain
CIBERONC, 28029, Madrid, Spain
Green Submitted, gold
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